Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls
Autor John T. Corthellen Limba Engleză Paperback – 16 apr 2014
- Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding
- Offers many details not present in other protocol books
- Describes relevant controls for each technique and what those controls mean
- Chapters include references (key articles, books, protocols) for additional study
- Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules
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Specificații
ISBN-13: 9780128014615
ISBN-10: 012801461X
Pagini: 124
Dimensiuni: 152 x 229 x 7 mm
Greutate: 0.18 kg
Ediția:New.
Editura: ELSEVIER SCIENCE
ISBN-10: 012801461X
Pagini: 124
Dimensiuni: 152 x 229 x 7 mm
Greutate: 0.18 kg
Ediția:New.
Editura: ELSEVIER SCIENCE
Cuprins
Introduction
General Notes
DNA and RNA Extraction Protocols
Agarose Gell Electrophoresis
Reverse Transcription (RT) and Polymerase Chain Reaction (PCR)
Antibodies and Titrations
Protein Extraction
Immunoblotting (Western Blot)
Immunoprecipitation
Perfusion and Immersion Fixation
Immunohistochemistry
In situ Hybridization
References
General Notes
DNA and RNA Extraction Protocols
Agarose Gell Electrophoresis
Reverse Transcription (RT) and Polymerase Chain Reaction (PCR)
Antibodies and Titrations
Protein Extraction
Immunoblotting (Western Blot)
Immunoprecipitation
Perfusion and Immersion Fixation
Immunohistochemistry
In situ Hybridization
References