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Basic Life Science Methods: A Laboratory Manual for Students and Researchers de Javeed Ahmad Tantray

Basic Life Science Methods: A Laboratory Manual for Students and Researchers

Autor Javeed Ahmad Tantray, Nighat Un Nissa, Rasy Fayaz Choh Wani, Sheikh Mansoor
en Limba Engleză Paperback – 21 sep 2022
Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.

  • Provides a practical resource on designing, executing and analyzing experiments and analytical procedures
  • Includes detailed and standardized coverage of basic research methods in the area
  • Presents step-by-step instructions on how to execute a large selection of life sciences experiments
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Specificații

ISBN-13: 9780443191749
ISBN-10: 0443191743
Pagini: 230
Dimensiuni: 191 x 235 mm
Greutate: 0.48 kg
Editura: ELSEVIER SCIENCE

Public țintă

Graduate students and early career researcher in life sciences.
Life sciences laboratory staff members looking for resources to update their practices.

Cuprins

1. Calculations of Molarity and Normality
2. Glassware washing and sterilization techniques
3. pH Meter: Its use and calibration
4. Principle and working of Autoclave
5. Principle and working of Centrifuge
6. Principles and working of Laminar
7. Estimation of Total cholesterol (Flegg 1972)
8. Estimation of Creatinine
9. Estimation of blood Urea
10. Estimation of blood glucose By GOD – POD method
11. Determination of Albumin, and Globulin in Plasma
12. Quantitative determination of Homocysteine
13. Estimation of serum bilirubin
14. Estimation of diagnostic enzyme ALT
15. Estimation of diagnostic enzyme AST
16. To Estimate Protein by Lowry’s Method
17. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method
18. To perform quantitative determination of total protein in Serum/plasma
19. Enzyme Activity Using Starch assay
20. To Estimate Protein by Bradford’s Assay
21. To Identify a Protein Sample Using SDS-PAGE
22. Genomic DNA isolation from whole blood
23. PCR for gene amplification
24. Agarose Gel Electrophoresis (AGE)
25. RNA Isolation from Human tissue
26. Real-time polymerase chain reaction (RT-qPCR)
27. Preparation of competent cells by CaCl2 treatment
28. RNA Isolation from Plant tissues
29. DNA isolation from the bacterial culture broth
30. DNA isolation from fungal Mycelium
31. Quantification of the DNA using spectrophotometer
32. Formaldehyde gel electrophoresis of the isolated RNA sample
33. Paper chromatography of different amino acid
34. Separation of plants pigments using Column Chromatography
35. Separation of plant pigment by Thin Layer Chromatography
36. Chromatin Immunoprecipitation (ChIP) Protocol
37. Types of Media
38. Preparation of Nutrient Agar Media
39. Serial Dilution of the Bacterial Cells
40. Spread Plate Method of The Bacterial Cells
41. Streak Plate Method
42. Identified Bacterial Colony in Culture Broth
43. Pour plate method for bacterial colony counting
44. Gram Staining of Bacteria
45. Casein hydrolysis test
46. Antibiotic resistance test by Agar Well Diffusion Method