This study aims to evaluate the chemical profile and fungicidal activity of the essential oil nanoemulsion obtained from Ba-har (Syrian pepper). The results obtained for the total phenolic content were quantified in 348.3 mg EAT g-1 and 346.21 mg EQ g-1 for flavonoids. The essential oil Ba-har was more efficient in inhibiting A niger as it had the lowest MCP (200 g mL-1), followed by P. chrysogenum (250 g mL-1) and then C. gloeosporioides (300 g mL-1). The identification of secondary metabolites present in Ba-har was quite considerable, since they are responsible for biological properties, thus inspiring the continuity of studies related to their biological activities. As for the total phenolic content and flavonoids present in the essential oil, they indicated the important antioxidant potential. The fungicidal potential of Ba-har oil presented strong inhibition and mortality, however the nanoemulsion product with the incorporated essential oil showed a more efficient action against the tested pathogenic fungi, being pointed out by this study as a new alternative in the control and combat of pathogenic fungi by means of the nanoemulsion product formulated with the oil.