Arginase: Structure, Function, and Therapeutic Targeting, Volume 740 in the Methods in Enzymology series, provides a comprehensive, methodology-driven perspective on arginase research spanning molecular, biochemical, and translational domains. Chapters in this new release include Expression, Purification, and Structural Characterization of Human and Leishmania Arginase with Comparative Analysis of Structures from the Protein Data Bank, Synthesis of First-Generation Arginase Inhibitors, Computational and Structure-Guided Design of Arginase Inhibitors, Metal Substitution in Arginase: Experimental Approaches to Study Metal-Dependent Catalysis, Therapeutic Arginase Formulations: PEGylation, Stability, and Advanced Delivery Systems, and much more.

Additional chapters cover Dual ARG1/ARG2 Inhibition: Experimental and Structural Strategies for Inhibitor Design and Optimization, Synthesis of Second-Generation Arginase Inhibitors, Cryo-EM Workflow and Structural Characterization of Arginase–Antibody Complexes, Development and Validation of an Electrochemical Assay for Arginase Activity, Experimental and Structural Analysis of H. pylori Arginase in Gastric Immune Evasion, Agmatinase and Alternative Arginine Utilization Pathways in Bacteria: Biochemical and Functional Perspectives, Catalytic Diversification of the Arginase Fold: Structural and Mechanistic Insights from DcsB, Beyond Arginase: Structural Biology of the Extended Guanidinase Family, Plant Arginase: Structural and Functional Roles in Nitrogen Metabolism, and much more.

• Provides comprehensive coverage of arginase from structure and mechanism to therapeutic targeting
• Includes methodology-driven chapters that emphasize experimental design, reproducibility, and analytical approaches
• Integrates academic and industry perspectives on inhibitor development, antibody-based and protein-based strategies, and translational applications