Antibody Engineering Protocols

SEQHUNT.- Molecular Modeling of Antibody-Combining Sites.- Structure and Properties of Human Immunoglobulin Light-Chain Dimers.- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins.- Detection of Human Variable Gene Family Expression at the Single-Cell Level.- Purification of Reduced and Alkylated Antibody Subunits.- Murine Monoclonal Antibody Development.- Comparative Properties of Polyclonal and Monoclonal Antibodies.- Evaluation of Antibody Clonality.- Affinity Immunoblotting.- Epitope and Idiotope Mapping Using Monoclonal Antibodies.- Anti-Idiotypic Antibodies That Mimic Opioids.- Catalytic Antibodies.- Preparation and Assay of Acetylcholinesterase Antibody.- DNA Hydrolysis by Antibodies.- Screening Strategies for Catalytic Antitransition-State Analog Antibodies.- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells.- Single-Chain Anti-DNA Fv.- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments.- Cloning and Bacterial Expression of an Esterolytic sFv.- Site-Directed Mutagenesis of Antibody-Variable Regions.- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation.- Chaperonins in Phage Display of Antibody Fragments.- Phage-Display Libraries of Murine and Human Antibody Fab Fragments.- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library.- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography.- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening.- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies.- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies.- Radiolabeling of Antibodies for Therapy and Diagnosis.