Genetic engineering is the study of the process of transforming genomes. It uses the elements and techniques of biotechnology to manipulate the genes and transfer them across organisms to develop better organisms and crops. The processes used in genetic engineering are gene isolation, molecular cloning, genetic transformation, etc. This book is a compilation of chapters that discuss the most vital concepts in the field of genetic engineering. It presents topics which include various methods and theories used in this vast field. Different approaches, evaluations and methodologies have been included in it. This textbook attempts to assist those with a goal of delving into the field of genetic engineering.