The book opens with an analysis of the methods commonly used for the immobilization of amylase on particles, the effect that the processes of adsorption and covalent immobilization have on the activity and stability of the enzyme, as well as on its stability and reusability. The authors go on to review current molecular strategies, including heterologous expression and mutations, for improving the production and properties of amylases from thermophilic bacteria. In conclusion, this compilation discusses the main advantages of the use of microorganisms for obtaining amylases: the production capacity in large quantities and ease of handling. Amylolytic enzymes have garnered attention because of their technological importance, economic benefits, and applicability in the commercial production of glucose.