The book offers a broad perspective, encompassing lucid descriptions of the process of affinity chromatography. Affinity chromatography covers a plethora of separation approaches that depend primarily on the use of biospecific interactions on the ligand for reversible adsorption of biomolecules and is one of the leading breakthroughs in the domain of bio-separation techniques. In this book, experts have attempted to present simplified fundamentals of this field, along with some remarkable applications of this diverse technique. There has been special focus on keeping the content concise and lucid. This work will garner significant attention along with delivering benefits and valuable data to the readers.