Vaccinia Virus and Poxvirology: Methods and Protocols (Methods in Molecular Biology, nr. 890)
Editat de Stuart N. Isaacsen Limba Engleză Paperback – 23 Aug 2016
Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.
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Specificații
ISBN-13: 9781493960835
ISBN-10: 1493960830
Pagini: 348
Dimensiuni: 178 x 254 x 18 mm
Greutate: 0.6 kg
Ediția: Softcover reprint of the original 2nd ed. 2012
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării: Totowa, NJ, United States
ISBN-10: 1493960830
Pagini: 348
Dimensiuni: 178 x 254 x 18 mm
Greutate: 0.6 kg
Ediția: Softcover reprint of the original 2nd ed. 2012
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării: Totowa, NJ, United States
Cuprins
1.
Working
Safely
with
Vaccinia
Virus:
Laboratory
Technique
and
Review
of
Published
Cases
of
Accidental
Laboratory
Infections
Stuart N. Isaacs
2. In-Fusion® Cloning with Vaccinia Virus DNA Polymerase
Chad R. Irwin, Andrew Farmer, David O. Willer, and David H. Evans
3. Genetic Manipulation of Poxviruses using Bacterial Artificial Chromosome Recombineering
Matthew G. Cottingham
4. Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA
Melanie Kremer, Asisa Volz, Joost Kreijtz, Michael H Lehmann, and Gerd Sutter
5. Isolation of Recombinant MVA using F13L Selection
Juana M. Sánchez-Puig, María M. Lorenzo, and Rafael Blasco
6. Screening for Vaccinia Virus Egress Inhibitors - Separation of IMV, IEV, and EEV
Chelsea M. Byrd and Dennis E. Hruby
7. Imaging of Vaccinia Virus Entry into HeLa Cells
Cheng-Yen Huang and Wen Chang
8. New Method for the Assessment of Molluscum Contagiosum Virus Infectivity
Subuhi Sherwani, Niamh Blythe, Laura Farleigh and Joachim J Bugert
9. An Intradermal Model for Vaccinia Virus Pathogenesis in Mice
Leon C. W. Lin, Stewart A. Smith and David C. Tscharke
10. Measurements of Vaccinia Virus Dissemination using Whole Body Imaging: Approaches for Predicting of Lethality in Challenge Models and Testing of Vaccines and Anti-viral Treatments
Marina Zaitseva, Senta Kapnick, and Hana Golding
11. Mousepox, a Small Animal Model of Smallpox
David Esteban, Scott Parker, Jill Schriewer, Hollyce Hartzler and R. Mark Buller
12. Analyzing CD8 T Cells in Mouse Models of Poxvirus Infection
Inge EA Flesch, Yik Chun Wong and David C. Tscharke
13. Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus
Xiangzhi Meng and Yan Xiang
14. Bioinformatics for Analysis of Poxvirus Genomes
Melissa Da Silva and Chris Upton
15. Antigen Presentation Assays to Investigate Uncharacterized Immunoregulatory Genes
Rachel L. Roper
16. Characterization of Poxvirus Encoded Proteins that Regulate Innate Immune Signaling Pathways
Florentina Rus, Kayla Morlock, Neal Silverman, Ngoc Pham, Girish J. Kotwal and William L. Marshall
17. Application of Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)
18. Central Nervous System Distribution of the Poxviral Proteins After Intranasal Administration of Proteins and Titering of Vaccinia Virus in the Brain after Intracranial Administration
Amod P. Kulkarni, Dhirendra Govender, Lauriston A. Kellaway, and Girish J. Kotwal
Stuart N. Isaacs
2. In-Fusion® Cloning with Vaccinia Virus DNA Polymerase
Chad R. Irwin, Andrew Farmer, David O. Willer, and David H. Evans
3. Genetic Manipulation of Poxviruses using Bacterial Artificial Chromosome Recombineering
Matthew G. Cottingham
4. Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA
Melanie Kremer, Asisa Volz, Joost Kreijtz, Michael H Lehmann, and Gerd Sutter
5. Isolation of Recombinant MVA using F13L Selection
Juana M. Sánchez-Puig, María M. Lorenzo, and Rafael Blasco
6. Screening for Vaccinia Virus Egress Inhibitors - Separation of IMV, IEV, and EEV
Chelsea M. Byrd and Dennis E. Hruby
7. Imaging of Vaccinia Virus Entry into HeLa Cells
Cheng-Yen Huang and Wen Chang
8. New Method for the Assessment of Molluscum Contagiosum Virus Infectivity
Subuhi Sherwani, Niamh Blythe, Laura Farleigh and Joachim J Bugert
9. An Intradermal Model for Vaccinia Virus Pathogenesis in Mice
Leon C. W. Lin, Stewart A. Smith and David C. Tscharke
10. Measurements of Vaccinia Virus Dissemination using Whole Body Imaging: Approaches for Predicting of Lethality in Challenge Models and Testing of Vaccines and Anti-viral Treatments
Marina Zaitseva, Senta Kapnick, and Hana Golding
11. Mousepox, a Small Animal Model of Smallpox
David Esteban, Scott Parker, Jill Schriewer, Hollyce Hartzler and R. Mark Buller
12. Analyzing CD8 T Cells in Mouse Models of Poxvirus Infection
Inge EA Flesch, Yik Chun Wong and David C. Tscharke
13. Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus
Xiangzhi Meng and Yan Xiang
14. Bioinformatics for Analysis of Poxvirus Genomes
Melissa Da Silva and Chris Upton
15. Antigen Presentation Assays to Investigate Uncharacterized Immunoregulatory Genes
Rachel L. Roper
16. Characterization of Poxvirus Encoded Proteins that Regulate Innate Immune Signaling Pathways
Florentina Rus, Kayla Morlock, Neal Silverman, Ngoc Pham, Girish J. Kotwal and William L. Marshall
17. Application of Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)
18. Central Nervous System Distribution of the Poxviral Proteins After Intranasal Administration of Proteins and Titering of Vaccinia Virus in the Brain after Intracranial Administration
Amod P. Kulkarni, Dhirendra Govender, Lauriston A. Kellaway, and Girish J. Kotwal
Textul de pe ultima copertă
Since
the
first
edition
of
Vaccinia
Virus
and
Poxvirology:
Methods
and
Protocols
was
published,
a
number
of
important
events
related
to
poxvirology
have
occurred,
such
as
FDA
approval
of
a
culture-based
live
smallpox
vaccine
and
the
vaccination
of
large
numbers
of
U.S.
military
personnel and
relatively
large
numbers
of
U.S.
civilians. Novel
anti-poxvirus
therapeutics
have
been
developed
and
have
been
used
in
emergency
settings.
The
second
edition
of
Vaccinia
Virus
and
Poxvirology
expands
upon
the
previous
edition
with
entirely
new
sets
of
protocols.
Written
in
the
highly
successful
Methods
in
Molecular
Biology™
series
format,
chapters
include
introductions
to
their
respective
topics,
lists
of
the
necessary
materials
and
reagents,
step-by-step,
readily
reproducible
laboratory
protocols,
and
key
tips
on
troubleshooting
and
avoiding
known
pitfalls.
Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.
Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.
Caracteristici
Details
the
most
current
poxvirus
protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Recenzii
From
the
foreword...
"The right book at the right time." -Bernard Moss
"...a collection of up-to-date protocols...the book is intended for virologists devoted to poxvirus research and scientists in vaccine research and some biotechnology area." - Chemotherapy
"The right book at the right time." -Bernard Moss
"...a collection of up-to-date protocols...the book is intended for virologists devoted to poxvirus research and scientists in vaccine research and some biotechnology area." - Chemotherapy