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Vaccinia Virus and Poxvirology: Methods and Protocols (Methods in Molecular Biology, nr. 890)

Editat de Stuart N. Isaacs
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en Limba Engleză Paperback – 23 Aug 2016
Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military and relatively large numbers of U.S. civilians. Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of Vaccinia Virus and Poxvirology expands upon the previous edition with entirely new sets of protocols. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.
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Specificații

ISBN-13: 9781493960835
ISBN-10: 1493960830
Pagini: 348
Dimensiuni: 178 x 254 x 18 mm
Greutate: 0.6 kg
Ediția: Softcover reprint of the original 2nd ed. 2012
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării: Totowa, NJ, United States

Cuprins

1. Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections
Stuart N. Isaacs
 2. In-Fusion® Cloning with Vaccinia Virus DNA Polymerase
Chad R. Irwin, Andrew Farmer, David O. Willer, and David H. Evans
 3. Genetic Manipulation of Poxviruses using Bacterial Artificial Chromosome Recombineering
Matthew G. Cottingham
 4. Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA
Melanie Kremer, Asisa Volz, Joost Kreijtz, Michael H Lehmann, and Gerd Sutter
 5. Isolation of Recombinant MVA using F13L Selection
Juana M. Sánchez-Puig, María M. Lorenzo, and Rafael Blasco
 6. Screening for Vaccinia Virus Egress Inhibitors - Separation of IMV, IEV, and EEV
Chelsea M. Byrd and Dennis E. Hruby
 7. Imaging of Vaccinia Virus Entry into HeLa Cells
Cheng-Yen Huang and Wen Chang
 8. New Method for the Assessment of Molluscum Contagiosum Virus Infectivity
Subuhi Sherwani, Niamh Blythe, Laura Farleigh and Joachim J Bugert
 9. An Intradermal Model for Vaccinia Virus Pathogenesis in Mice
Leon C. W. Lin, Stewart A. Smith and David C. Tscharke
 10. Measurements of Vaccinia Virus Dissemination using Whole Body Imaging: Approaches for Predicting of Lethality in Challenge Models and Testing of Vaccines and Anti-viral Treatments
Marina Zaitseva, Senta Kapnick, and Hana Golding
 11. Mousepox, a Small Animal Model of Smallpox
David Esteban, Scott Parker, Jill Schriewer, Hollyce Hartzler and R. Mark Buller
 12. Analyzing CD8 T Cells in Mouse Models of Poxvirus Infection
Inge EA Flesch, Yik Chun Wong and David C. Tscharke
 13. Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus
Xiangzhi Meng and Yan Xiang
 14. Bioinformatics for Analysis of Poxvirus Genomes
Melissa Da Silva and Chris Upton 
15. Antigen Presentation Assays to Investigate Uncharacterized Immunoregulatory Genes
Rachel L. Roper
16. Characterization of Poxvirus Encoded Proteins that Regulate Innate Immune Signaling Pathways
Florentina Rus, Kayla Morlock, Neal Silverman, Ngoc Pham, Girish J. Kotwal and William L. Marshall
17. Application of Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)
18. Central Nervous System Distribution of the Poxviral Proteins After Intranasal Administration of Proteins and Titering of Vaccinia Virus in the Brain after Intracranial Administration
Amod P. Kulkarni, Dhirendra Govender, Lauriston A. Kellaway, and Girish J. Kotwal

Textul de pe ultima copertă

Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military personnel and relatively large numbers of U.S. civilians. Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of Vaccinia Virus and Poxvirology expands upon the previous edition with entirely new sets of protocols. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.

Caracteristici

Details the most current poxvirus protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts

Recenzii

From the foreword...

"The right book at the right time." -Bernard Moss

"...a collection of up-to-date protocols...the book is intended for virologists devoted to poxvirus research and scientists in vaccine research and some biotechnology area." - Chemotherapy