Cantitate/Preț
Produs

The Blood-Brain Barrier: Methods and Protocols (Methods in Molecular Biology, nr. 2492)

Editat de Nicole Stone
Notă GoodReads:
en Limba Engleză Hardback – 24 Jun 2022
This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
Citește tot Restrânge

Din seria Methods in Molecular Biology

Preț: 91232 lei

Preț vechi: 96034 lei
-5%

Puncte Express: 1368

Preț estimativ în valută:
17575 16911$ 15652£

Carte tipărită la comandă

Livrare economică 16-30 noiembrie
Livrare express 08-18 octombrie pentru 56426 lei

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781071622889
ISBN-10: 1071622889
Ilustrații: XI, 357 p. 83 illus., 73 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.86 kg
Ediția: 1st ed. 2022
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării: New York, NY, United States

Cuprins

Part I: Introduction to Blood-Brain Barrier (BBB) Physiology
 
1. Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease
            Heather L. McConnell and Anusha Mishra
 
2. In Vitro Models of the Blood-Brain Barrier
            Snehal Raut, Aditya Bhalerao, Behnam Noorani, and Luca Cucullo
 
Part II: Using Pluripotent Stem Cells in Models of the BBB
 
3. In Vitro Models of the Human Blood-Brain Barrier Based on Human Induced Pluripotent Stem Cells: Opportunities and Challenges
            Iqra Pervaiz and Abraham J. Al-Ahmad
 
4. Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study Bacterial–Brain Endothelial Cell Interactions
            Eric R. Espinal, S. Jerod Sharp, and Brandon J. Kim
 
5. An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier
            Mary Goodwin-Trotman, Krushangi Patel, and Alessandra Granata
 
6. A Three-Dimensional Brain-on-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes
            Lumei Liu, Youngmi Koo, Teal Russell, and Yeoheung Yun
 
Part III: Co-Culture, Permeability, and Transwell Models of the BBB: Methods for Studying Shear Stress, Barrier Integrity and Breakdown
 
7. An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies
            Adjanie Patabendige
 
8. Establishment of an In Vitro Model of Human Blood-Brain Barrier to Study the Impact of Ischaemic Injury
            Rais Reskiawan A. Kadir, Mansour Alwjwaj, and Ulvi Bayraktutan
 
9. A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier
            Patricia Miranda-Azpiazu and Sikha Saha  
10. A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress
            Gemma Molins Gutierrez, Jordi Martorell, Antonio G. Salazar-Martin, and Mercedes Balcells
 
Part IV: Microfluidic and Chip Models of the BBB
 
11. Novel, Emerging Chip Models of the BBB and Future Directions
            Paul M. Holloway
 
12. Integrating Primary Astrocytes in a Microfluidic Model of the Blood-Brain Barrier
            Eliana Lauranzano, Marco Rasile, and Michela Matteoli
 
13. Fabrication of Microtube-Embedded Chip to Mimic Blood-Brain Barrier Capillary Vessels
            Dilshan Sooriyaarachchi, Shahrima Maharubin, and George Z. Tan
 
14. BBB-on-a-Chip: Modeling Functional Human BBB by Mimicking 3D Brain Angiogenesis Using Microfluidic Chip
            Somin Lee, Minhwan Chung, and Noo Li Jeon
 
Part V: Models to Study Specific Pathologies at the BBB: Cancer, Neurodegeneration, and More
 
15. Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability
            Cláudia Martins and Bruno Sarmento
 
16. An In Vitro Human Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis
            Caroline Mysiorek, Lucie Dehouck, Fabien Gosselet, and Marie-Pierre Dehouck
 
17. An In Vivo Mouse Model to Study Blood-Brain Barrier Destabilization in the Chronic Phase of Stroke
            Svetlana M. Stamatovic, Chelsea M. Phillips, Richard F. Keep, and Anuska V. Andjelkovic
  18. Immunohistochemical Analysis of Tight Junction Proteins
            Chris Greene and Matthew Campbell
 
19. An In Vitro BBB Model to Study Firm Shear-Stress Resistant Leukocyte Adhesion to Human Brain Endothelial Cells
            Camilla Cerutti and Ignacio A. Romero
 
20. An In Vitro Model of the Blood-Brain Barrier (BBB) to Study Alzheimer's Disease: The Role of β-Amyloid and Its Influence on PBMC Infiltration
            Simona Federica Spampinato, Yukio Takeshita, and Birgit Obermeier

Textul de pe ultima copertă

This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.

Caracteristici

Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts