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Technology Platforms for 3D Cell Culture: A User′s Guide

Editat de Stefan Przyborski
Notă GoodReads:
en Limba Engleză Carte Hardback – 21 Apr 2017
With the recognition that cells live in complex, three–dimensional (3D) tissue, it should come as no surprise that many laboratories are transitioning from conventional two–dimensional (2D) cell culture in Petri dishes to 3D cell culture techniques but where to begin? Technology Platforms for 3D Cell Culture: A User s Guide points to the current options available to perform 3D culture, shows where such technology is available, explains how it works and reveals how it can be used by scientists working in their own labs.
Featuring contributions from leading developers and researchers at the cutting edge of today s 3D cell technology, the chapters present a comprehensive, focused guide to the current state–of–the–art technologies available for 3D cell culture, along with clear instructions and guidance on performing specific 3D culture methods. Colour illustrations are included, along with examples of where such technologies have been successfully applied, information on resources and technical support to help initiate the use of 3D culture methods.
Technology Platforms for 3D Cell Culture: A User s Guide is an indispensable single–volume resource for students, laboratory–based scientists and cell biologists seeking knowledge of the state–of–the–art technologies currently available for 3D cell culture.
www.wiley.com/wiley–blackwell
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Specificații

ISBN-13: 9781118851500
ISBN-10: 1118851501
Pagini: 424
Dimensiuni: 171 x 250 x 25 mm
Greutate: 0.97 kg
Editura: Wiley
Locul publicării: Chichester, United Kingdom

Public țintă

Post–graduate level students for courses in cell biology, and scientists and cell biologists currently practising conventional 2D cell culture; also for managers of cell culture facilities / departments, R&D heads of section, technical supervisors, advisors, etc. who would recommend methods to perform

Textul de pe ultima copertă

With the recognition that cells live in complex, three–dimensional (3D) tissue, it should come as no surprise that many laboratories are transitioning from conventional two–dimensional (2D) cell culture in Petri dishes to 3D cell culture techniques but where to begin? Technology Platforms for 3D Cell Culture: A User s Guide points to the current options available to perform 3D culture, shows where such technology is available, explains how it works and reveals how it can be used by scientists working in their own labs.
Featuring contributions from leading developers and researchers at the cutting edge of today s 3D cell technology, the chapters present a comprehensive, focused guide to the current state–of–the–art technologies available for 3D cell culture, along with clear instructions and guidance on performing specific 3D culture methods. Colour illustrations are included, along with examples of where such technologies have been successfully applied, information on resources and technical support to help initiate the use of 3D culture methods.
Technology Platforms for 3D Cell Culture: A User s Guide is an indispensable single–volume resource for students, laboratory–based scientists and cell biologists seeking knowledge of the state–of–the–art technologies currently available for 3D cell culture.
www.wiley.com/wiley–blackwell

Cuprins

List of contributors
Preface
List of abbreviations
1 An introduction to the third dimension for routine cell culture, 1
Antonio Romo ]Morales and Stefan Przyborski
Part I: Aggregate ]based technologies
2 Gravity ]enforced microtissue engineering
Randy Strube, Johannes Haugstetter, Markus Furter, Andreia Fernandez, David Fluri and Jens M. Kelm
3 Physiologically relevant spheroid models for three ]dimensional cell culture
Nicole A. Slawny and MaryAnn Labant
4 NanoCulture Plate: A scaffold ]based high ]throughput three ]dimensional cell culture system suitable for live imaging and co ]culture
Manabu Itoh, Kazuya Arai, Hiromi Miura and M. Mamunur Rahman
5 Micro ]moulded non ]adhesive hydrogels to form multicellular microtissues the 3D Petri DishR
Elizabeth Leary, Sean Curran, Michael Susienka, Kali L. Manning, Andrew M. Blakely and Jeffrey R. Morgan
6 Organotypic microtissues on an air ]liquid interface
Lars E. Sundstrom, Igor Charvet and Luc Stoppini
Part II: Hydrogels
7 Materials and assay systems used for three ]dimensional cell culture
Suparna Sanyal and Marshall Kosovsky
8 HyStemR, a customisable hyaluronan ]based hydrogel matrix for 3D cell culture
T. I. Zarembinski, B. J. Engel, N. J. Doty, P. E. Constantinou, M. V. Onorato, I. E. Erickson, E. L. S. Fong, M. Martinez, R. L. Milton, B. P. Danysh, N. A. Delk, D. A. Harrington, M. C. Farach ]Carson and D. D. Carson
9 3 ]D Life biomimetic hydrogels: A modular system for cell environment design
Brigitte M. Angres and Helmut Wurst
Part III: Scaffolds
10 AlvetexR, a highly porous polystyrene scaffold for routine three ]dimensional cell culture
Antonio Romo ]Morales, Eleanor Knight and Stefan Przyborski
11 CelluSponge and Go Matrix as innovative three ]dimensional cell culture platforms
Bramasta Nugraha
12 MimetixR electrospun scaffold: An easy ]to ]use tool for 3D cell culture in drug discovery and regenerative medicine
Robert J. McKean and Elena Heister
Part IV: 3D bioreactor technologies
13 Quasi VivoR bioreactor technology
J. Malcolm Wilkinson
14 Three ]dimensional cell ]based assays in hollow fibre bioreactors
John J. S. Cadwell and William G. Whitford
15 Three ]dimensional engineered tissues for high ]throughput compound screening: Mechanical properties of skin and ageing
Michael Conway, Ayla Annac and Tetsuro Wakatsuki
16 Three ]dimensional cell culture in the Rotary Cell Culture System
Stephen S. Navran
Glossary
Index

Notă biografică

Stefan Przyborski is Professor of Cell Technology in the Department of Biosciences, Durham University, UK. He is also the Chief Scientific Officer for ReproCELL Europe, UK. A recognised leader in the field of 3D cell culture, Professor Przyborski has published extensively in this field and presents his work regularly at international events.