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Post-Transcriptional Gene Regulation: Methods in Molecular Biology, cartea 1358

Editat de Erik Dassi
en Limba Engleză Paperback – 23 aug 2016
This volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level. Post-Transcriptional Gene Regulation, Second Edition is organized in six sections describing bioinformatics approaches for studying post-transcriptional regulation, various expression profiling approaches, protein-RNA interaction and non-coding RNA investigation techniques, methods for profiling RNA modifications, and other techniques such as alternative translation initiation or polyadenylation sites determination. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Post-Transcriptional Gene Regulation, Second Edition  is a versatile resource to researchers studying post-transcriptional regulation, introducing the most recent techniques and providing a comprehensive guide to their implementation.
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Specificații

ISBN-13: 9781493949694
ISBN-10: 1493949691
Pagini: 353
Ilustrații: XIII, 353 p. 43 illus., 30 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 7.73 kg
Ediția:Softcover reprint of the original 2nd ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

PART I: Bioinformatics
1. Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression
Alessandro Quattrone and Erik Dassi
2. A Computational Approach for the Discovery of Protein-RNA Networks
Domenica Marchese, Carmen Maria Livi, and Gian Gaetano Tartaglia
PART II: Expression Studies
3. Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells
Hannah Pham, Nicola A. Kearns, and René Maehr
 
4. Studying the Translatome with Polysome Profiling
Paola Zuccotti and Angelika Modelska
           
5. Exploring Ribosome Positioning on Translating Transcripts with Ribosome Profiling
Pieter Spealman, Hao Wang, Gemma May, Carl Kingsford, and C. Joel Mcmanus
           
6. Studying Isoform-Specific Recruitment to Polyribosomes with Frac-Seq
Rocio T. Martinez-Nunez and Jeremy Sanford
           
7. Use of the Pbutr Reporter System for Scalable Analysis of 3' UTR-Mediated Gene Regulation
Arindam Chaudhury and Joel R. Neilson
 
 
PART III: RBP Interactomics
 
8. Comprehensive Identification of RNA-Binding Proteins by RNA Interactome Capture
Alfredo Castello, Rastislav Horos, Claudia Strein, Bernd Fischer, Katrin Eichelbaum, Lars M. Steinmetz, Jeroen Krijgsveld, and Matthias W. Hentze
 
9. Identifying RBP Targets with RIP-Seq
Hans-Herman Wessels, Antje Hirsekorn, Uwe Ohler, and Neelanjan Mukherjee
   
10. PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites
Charles Danan, Sudhir Manickavel, and Markus Hafner
   
11. Profiling the Binding Sites of RNA-Binding Proteins with Nucleotide Resolution Using Iclip
FX Reymond Sutandy, Andrea Hildebrandt, and Julian König
   
12. A Pipeline for PAR-CLIP Data Analysis
Marvin Jens
 
 
PART IV: Non-Coding RNAs
 
13. Capture and Identification Of Mirna Targets By Biotin Pulldown And RNA-Seq
Shen Mynn Tan and Judy Lieberman
   
14. Identification of Mirna-Target RNA Interactions Using CLASH
Alexandra Helwak and David Tollervey
 
 
PART V:  RNA Modifications
 
15. Genome-Wide Analysis of A-To-I RNA Editing 
Yiannis A. Savva, Georges St. Laurent, and Robert A. Reenan
   
16. Nucleotide-Level Profiling Of M5c RNA Methylation
Tennille Sibbritt, Andrew Shafik, Susan J. Clark, and Thomas Preiss
   
17. Probing N6-Methyladenosine (M6a) RNA Modification in Total RNA with SCARLET
Nian Liu and Tao Pan
 
 
PART VI: Other Aspects Of PTR
    
18. Genome-Wide Identification of Alternative Polyadenylation Events Using 3’T-Fill
Stefan Wilkening, Vicent Pelechano, and Lars M. Steinmetz
 
19. Genome-Wide Profiling Of Alternative Translation Initiation Sites
Xiangwei Gao, Ji Wan, and Shu-Bing Qian
 
20. Genome-Wide Study Of Mrna Isoform Half-Lives  
Joseph V. Geisberg and Zarmik Moqtaderi
 
21. Visualizing Mrna Dynamics in Live Neurons and Brain Tissues
Hye Yoon Park and Minho Song
 
22. Single Molecule Live-Cell Visualization of Pre-Mrna Splicing
Robert M. Martin, José Rino, Ana C. De Jesus, and Maria Carmo-Fonseca
 
 

Textul de pe ultima copertă

This volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level. Post-Transcriptional Gene Regulation, Second Edition is organized in six sections describing bioinformatics approaches for studying post-transcriptional regulation, various expression profiling approaches, protein-RNA interaction and non-coding RNA investigation techniques, methods for profiling RNA modifications, and other techniques such as alternative translation initiation or polyadenylation sites determination. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Post-Transcriptional Gene Regulation, Second Edition  is a versatile resource to researchers studying post-transcriptional regulation, introducing the most recent techniques and providing a comprehensive guide to their implementation.

Caracteristici

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts