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In Vitro Mutagenesis Protocols: Third Edition (Methods in Molecular Biology, nr. 634)

Editat de Jeff Braman
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en Limba Engleză Paperback – 23 Aug 2016
In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.
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Specificații

ISBN-13: 9781493956661
ISBN-10: 1493956663
Pagini: 442
Ilustrații: XIV, 442 p.
Dimensiuni: 178 x 254 mm
Greutate: 1.17 kg
Ediția: Softcover reprint of the original 3rd ed. 2010
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării: Totowa, NJ, United States

Cuprins

Part I: Mutagenesis in Various Microbial Backgrounds1. Mutagenesis Protocols in Saccharomyces cerevisiae by in vivo Overlap ExtensionMiguel Alcalde2. In vitro Mutagenesis of Brucella SpeciesThomas A. Ficht, Jianwu Pei, and Melissa Kahl-McDonagh3. Random Mutagenesis Strategies for Campylobacter and Helicobacter SpeciesDuncan J.H. Gaskin and Arnoud H.M. van Vliet4. Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes (BAC)Yuguang Zhao and Venugopal Nair5. An Efficient Protocol for VZV BAC-Based MutagenesisZhen Zhang, Ying Huang, and Hua Zhu6. A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxb1 IntegraseSophie H. Adjalley, Marcus C.S. Lee, and David A. FidockPart II: PCR Mutagenesis7. Random Mutagenesis by Error-Prone PCRElizabeth O. McCullum, Berea A.R. Williams, Jinglei Zhang, and John C. Chaput8. A Rapid and Versatile PCR-Based Site-Directed Mutagenesis Protocol for Generation of Mutations Along the Entire Length of a Cloned cDNAVincent Dammai9. Rapid Sequence Scanning Mutagenesis Using in silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP)Ulrich Krauss, Karl-Erich Jaeger, and Thorsten Eggert10. Insertion and Deletion Mutagenesis by Overlap Extension PCRJehan Lee, Myeong-Kyun Shin, Dong-Kyun Ryu, Seahee Kim, and Wang-Shick Ryu11. Targeted Amplification of Mutant Strands for Efficient Site-Directed Mutagenesis and Mutant ScreeningLei Young and Qihan Dong12. A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated ProteinsOran Erster and Moti Liscovitch13. Amplification of Orthologous Genes Using Degenerate PrimersSamya Chakravorty and Jim O. VigoreauxPart III: Reviews14. Computational Evaluation of Protein Stability Change Upon MutationsShuangye Yin, Feng Ding, and Nikolay V. Dokholyan15. Approaches for Using Animal Models to Identify Loci that Genetically Interact with Human Disease-Causing Point MutationsJosef D. FrankePart IV: Protein Evolution Mutagenesis16. Using Peptide Loop Insertion Mutagenesis for the Evolution of ProteinsChristian Heinis and Kai Johnsson 17. Massive Mutagenesis®: High-Throughput Combinatorial Site-Directed MutagenesisJulien Sylvestre18. Directed in vitro Evolution of Reporter Genes Based on Semi-Rational Design and High Throughput ScreeningAi-Sheng Xiong, Quan-Hong Yao, Ri-He Peng, and Zong-Ming Cheng19. Ribosome Display for Rapid Protein Evolution by Consecutive Rounds of Mutation and SelectionHayato Yanagida, Tomoaki Matsuura, and Tetsuya YomoPart V: Protein Structure and Function Mutagenesis20. Fine-Tuning Enzyme Activity through Saturation MutagenesisHolly H. Hogrefe21. Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling PropertiesDanijela Markovic and Dimitris K. Grammatopoulos22. Site-Directed Mutagenesis for Improving Biophysical Properties of VH DomainsMehdi Arbabi-Ghahroudi, Roger MacKenzie, and Jamshid Tanha23. Phenotype Based Functional Gene Screening Using Retrovirus-Mediated Gene Trapping in Quasi-Haploid RAW 264.7 CellsSung Ouk Kim and Soon-Duck Ha24. Site-Directed Disulfide Cross-Linking to Probe Conformational Changes of a

Recenzii

From the reviews of the third edition:
“This well-illustrated book details specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein-structure function, protein regulation of biological processes, and protein activity and mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. … Scientists, students, and others interested in the field of in vitro mutagenesis will find this extremely useful. … This book successfully describes a variety of in vitro mutagenesis protocols for scientists working in this area.” (Omer Iqbal, Doody’s Review Service, November, 2010)

Textul de pe ultima copertă

In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.

Caracteristici

Establishes protein structure, function, activity, and interrelationships by authors of peer reviewed articles published in respected journals
Covers computational methods to evaluate protein stability and the resultant change upon introducing mutations
Utilizes animal models to identify loci that genetically interact with human disease-causing point mutations
Includes detailed methods on protein structure and function mutagenesis
Includes supplementary material: sn.pub/extras