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Immunohistochemistry and Immunocytochemistry: Essential Methods (Essential Methods)

Editat de Simon Renshaw
Notă GoodReads:
en Limba Engleză Carte Paperback – 13 Jan 2017
Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount.
Immunohistochemistry and Immunocytochemistry: Essential Methods is the second edition of Immunohistochemisrty: Methods Express, previously published by Scion Publishing. The book is a comprehensive research guide that describes the methodologies of immunochemistry. Each chapter discusses the merits and limitations of the various approaches and then provides selected tried–and–tested protocols, as well as a plethora of good theoretical information and practical advice, for immediate use at the bench. Subjects covered include, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy.
This book offers a wealth of knowledge to the novice immunochemist, who wishes to understand fully the theory and practice of immunochemical staining techniques and obtain reliable and reproducible data time and time again. For the experienced immunochemist, it is a comprehensive reference guide, allowing optimization of existing immunochemical staining protocols and a resource to further increase knowledge and understanding.
About the Editor
Simon Renshaw, Abcam plc, Cambridge, UK
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Specificații

ISBN-13: 9781118717776
ISBN-10: 1118717775
Pagini: 248
Dimensiuni: 160 x 244 x 13 mm
Greutate: 0.52 kg
Ediția: 2nd Edition
Editura: Wiley
Seria Essential Methods

Locul publicării: Chichester, United Kingdom

Public țintă

This book offers a wealth of knowledge to the novice immunochemist, who wishes to understand fully the theory and practice of immunochemical staining techniques and obtain reliable and reproducible data time and time again. For the experienced immunochemist, it is a comprehensive reference guide, allowing optimization of existing immunochemical staining protocols and a resource to further increase knowledge and understanding.
First edition sold in bulk to AbCam – opportunities to do the same for second edition.

Textul de pe ultima copertă

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount.
Immunohistochemistry and Immunocytochemistry: Essential Methods is the second edition of Immunohistochemisrty: Methods Express, previously published by Scion Publishing. The book is a comprehensive research guide that describes the methodologies of immunochemistry. Each chapter discusses the merits and limitations of the various approaches and then provides selected tried–and–tested protocols, as well as a plethora of good theoretical information and practical advice, for immediate use at the bench. Subjects covered include, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy.
This book offers a wealth of knowledge to the novice immunochemist, who wishes to understand fully the theory and practice of immunochemical staining techniques and obtain reliable and reproducible data time and time again. For the experienced immunochemist, it is a comprehensive reference guide, allowing optimization of existing immunochemical staining protocols and a resource to further increase knowledge and understanding.
About the Editor
Simon Renshaw, Abcam plc, Cambridge, UK

Cuprins

List of Contributors vii Preface ix
Acknowledgements xi
Chapter 1: Antibodies for Immunochemistry 1
Mark Cooper and Sheriden Lummas
Introduction 1
Immunogens for Antibody Production 5
Antibody Production 12
Antibody Purification 16
Fragment Antibody Preparations 20
Antibody Labelling 21
Antibody Stability and Storage 23
References 24
Chapter 2: The Selection of Reporter Labels 25
Judith Langenick
Introduction 25
Enzymatic Labels 26
Fluorescence Detection 29
References 32
Chapter 3: Immunohistochemistry and Immunocytochemistry 35
Simon Renshaw
Specimen Formats for Immunochemistry 36
Fixation 37
Processing Tissue Blocks to Paraffin Wax 46
Microtomy 47
Tissue Microarrays 47
Specimen Storage 48
Decalcification 49
Antigen Retrieval 50
Controls 56
Immunochemical Staining Techniques (Optimizing a New Antibody) 57
Counterstains 71
Mounting 74
Troubleshooting 76
Examples of Immunostaining Photomicrographs 76
Acknowledgements 101
References 101
Chapter 4: Multiple Immunochemical Staining Techniques 103
Sofia Koch
Introduction 103
Methods and Approaches 115
References 122
Chapter 5: Quality Assurance in Immunochemistry 123
Peter Jackson and Michael Gandy
Introduction 123
Methods and Approaches 125
Automated Immunochemical Staining 147
Troubleshooting 149
References 154
Chapter 6: Automated Immunochemistry 157
Emanuel Schenck and Simon Renshaw
Introduction 157
Methods and Approaches 160
Other Forms of Automation 164
References 168
Chapter 7: Confocal Microscopy 169
Ann Wheeler
Introduction 169
When Should Confocal be Used? 173
Applications: For Example Co–localization, Quantification, 3D Visualization and Kinetics 173
How To Set Up a Confocal Experiment? 174
References 198
Further Readings 198
Chapter 8: Ultrastructural Immunochemistry 199
Jeremy Skepper and Janet Powell
Introduction 199
Methods and Approaches 207
References 222
Index 227