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Detection of Tuberculosis By Polymerase Chain Reaction (PCR)

Autor Hymonti Dey, Sudip Debnath
en Limba Engleză Paperback – 20 iun 2012
The polymerase chain reaction (PCR) has recently been investigated for detecting Mycobacterium tuberculosis in clinical specimens and appears to have significant potential as a rapid, sensitive, and specific assay for diagnosing infections caused by this organism. One of the remaining issues regarding the use of this technique is the extraction of DNA from clinical material prior to performing the PCR. It requires the use of DNA extraction method that can efficiently lyse mycobacterial cells and recover small amounts of DNA. The method of heating the sample in a boiling water bath to break down the bacterial cell wall and to release the DNA was compared with that of enzymatic lysis of bacteria and then phenol-chloroform extraction of DNA (Modified chemical method). Modified chemical method was better than just boiling the sample to remove DNA for amplification with PCR. In this study 140 sputum samples were collected from suspected prisoners at Dhaka Central Jail. All of those samples were prepared by two DNA extraction methods and examined by PCR, and the results were compared with the results of acid-fast stained smears and cultures.
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Specificații

ISBN-13: 9783659161445
ISBN-10: 3659161446
Pagini: 104
Dimensiuni: 152 x 229 x 6 mm
Greutate: 0.16 kg
Editura: LAP LAMBERT ACADEMIC PUBLISHING AG & CO KG
Colecția LAP Lambert Academic Publishing

Notă biografică

Hymonti Dey, is a promising Research Officer presently working in the Tuberculosis Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B). She did her MS in Biochemistry and Molecular Biology from University of Dhaka, Bangladesh. She has significant skills on PCR technology.